Nara – mechentel news – A. Dota et al. from the Ophthalmic Research and Development Center, Santen Pharmaceutical Co, Ltd, Ikoma-shi, Nara, Japan, reported in the issue of january fro Clin. Ophthalmol. that Gefarnate may be effective for treating dry eye. The aim of this study was to evaluate the effect of gefarnate on mucin-like glycoprotein secretion in isolated rabbit conjunctival tissue, and on corneal epithelial damage in rabbit and cat dry-eye models. The scientists treated isolated conjunctival tissue from rabbits with gefarnate. They detected Mucin-like glycoprotein in the culture supernatant by an enzyme-linked lectin assay. Gefarnate ointment was topically applied to eyes once daily for 7 days in the rabbit dry-eye model, in which the lacrimal glands, Harderian gland, and nictitating membrane were removed, or for 4 weeks in the cat dry-eye model, in which the lacrimal gland and nictitating membrane were removed. Corneal epithelial damage was evaluated by measurement of corneal permeability by rose bengal in the rabbit model or by fluorescein staining in the cat model. Gefarnate stimulated mucin-like glycoprotein secretion in conjunctival tissue in a dose-dependent manner. In the rabbit dry-eye model, application of gefarnate ointment to the eyes resulted in a dose-dependent decrease in rose bengal permeability in the cornea, with the effect being significant at concentrations of ≥0.3%. In the cat dry-eye model, application of gefarnate ointment resulted in a significant decrease in the corneal fluorescein staining score. These results suggest that gefarnate stimulates in vitro secretion of mucin-like glycoprotein in conjunctival tissue and ameliorates corneal epithelial damage in animal dry-eye models.
Autoren: Dota A, Takaoka-Shichijo Y, Nakamura M. Korrespondenz: Atsuyoshi Dota, Ophthalmic Research and Development Center, Santen Pharmaceutical Co, Ltd, 8916-16 Takayama-cho, Ikoma-shi, Nara 630-0101, Japan. E-Mail: firstname.lastname@example.org. Studie: Gefarnate stimulates mucin-like glycoprotein secretion in conjunctival tissue and ameliorates corneal epithelial damage in animal dry-eye models. Quelle: Clin Ophthalmol. 2013;7:211-7. doi: 10.2147/OPTH.S39061. Web: http://www.dovepress.com/gefarnate-stimulates-mucin-like-glycoprotein-secretion-in-conjunctival-peer-reviewed-article-OPTH