Ottawa – mechentel news – We present an optimized surgical technique for feline retinal detachment which allows for natural re-attachment, reduces retinal scarring and vitreal bands, and allows central placement of the detachment in close proximity to the optic nerve. This enables imaging via Optical Coherence Tomography (OCT) and multifocal electroretinography (mfERG) analysis. Ideal detachment conditions involve a lensectomy followed by a three-port pars plana vitrectomy. A 16-20 % retinal detachment is induced by injecting 8 % C3F8 gas into the subretinal space in the central retina with a 42G cannula. The retinal detachment resolves approximately 6 weeks post-surgery. Imaging is enhanced by using a 7.5 and 20 diopter lens for OCT and mfERG fundus imaging, respectively, to compensate for the removed lens.
Autors: Wassmer S, Leonard BC, Coupland SG, Baker A, Hamilton J, Torlone R, Zacks DN, Tsilfidis C. Correspondence: Ottawa Hospital Research Institute, Regenerative Medicine, K1H 8L6, Ottawa, ON, Canada. E-Mail: email@example.com. Study: The Development of a Cat Model of Retinal Detachment and Re-attachment. Source: Adv Exp Med Biol. 2016;854:315-21. doi: 10.1007/978-3-319-17121-0_42. Web: http://link.springer.com/chapter/10.1007%2F978-3-319-17121-0_42.
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